Journal: Veterinary Microbiology

Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

doi: 10.1016/j.vetmic.2020.108793

Figure Lengend Snippet: Primers used for genes amplication.

Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

Techniques: Quantitative RT-PCR, Amplification

Journal: Veterinary Microbiology

Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

doi: 10.1016/j.vetmic.2020.108793

Figure Lengend Snippet: Identification of proteins that interact with PLP1 . HEK293 T cells were seeded in a six-well plate and cotransfected with pCMV-PLP1-HA, pECMV-3×FLAG-PCBP1, pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, cells were lysed and immunoprecipitated with mouse anti-FLAG antibody. Immunoprecipitated samples were analyzed by western blotting.

Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

Techniques: Plasmid Preparation, Immunoprecipitation, Western Blot

Journal: Veterinary Microbiology

Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

doi: 10.1016/j.vetmic.2020.108793

Figure Lengend Snippet: Co-localization of PCBP2 with PLP1 . (A) Vero E6 cells were cotransfected with pCMV-PLP1-HA and pECMV-3×FLAG-PCBP2 or empty pCMV-HA vector. The cells were assessed using an indirect immunofluorescence assay with anti-HA and anti-FLAG antibodies. (B) Co-localization of PLP1 and endogenous PCBP2 in Vero E6 cells. The cells were transfected with pCMV-PLPL-HA or empty pCMV-HA vector and then subjected to the IFA with anti-HA and anti-PCBP2 antibodies.

Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

Techniques: Plasmid Preparation, Immunofluorescence, Transfection

Journal: Veterinary Microbiology

Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

doi: 10.1016/j.vetmic.2020.108793

Figure Lengend Snippet: PLP1 up-regulates PCBP2 expression . Vero 6 cells were transfected with different concentrations of pCMV-PLP1-HA or empty pCMV-HA vector and western blotting was performed using anti-HA, anti-PCBP2 and anti-tubulin antibodies.

Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot

Journal: Veterinary Microbiology

Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

doi: 10.1016/j.vetmic.2020.108793

Figure Lengend Snippet: PCBP2 affects PEDV infection . IPEC-J2 cells were transfected with three different PCBP2-specific siRNAs. After 24 h, PCBP2 protein expression was assessed by western blotting with anti-PCBP2 and anti-tubulin antibodies. (B to C) IPEC-J2 cells were transfected with siRNA1, then 24 h later infected with PEDV for 24 h and 48 h. PCBP2 mRNA levels and PEDV RNA levels were determined by quantitative RT-PCR. (D) IPEC-J2 cells were transfected with pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, the cells were infected with PEDV at a MOI of 0.1. PEDV viral loads were determined at 24, 48 and 60 hpi by quantitative RT-PCR.

Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

Techniques: Infection, Transfection, Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation